FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16019055
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA11400.023.546173145
AGATTAT114350.015.607107145
CGCCGTT11602.2009772E-1011.257397145
GTAGATA69450.011.07279145
CGTGTAT19650.010.706781145
CGTATCA291300.09.364203145
GTCGCCG417600.08.616774145
GCCGTAT335200.08.505738145
GTATCAT341350.08.352493145
ATCATTA318250.08.206492145
CCGTATC311450.08.167015140-144
AGGGGGG182250.08.120563145
GGTCGCC442550.07.967047145
TATTGCG14601.0308504E-67.93898347
AAGGGGG193200.07.8480663145
TCGTGTT66650.07.837108145
TGGTCGC453650.07.724132145
TATCATT343050.07.5286317145
CGCCGTA405600.07.4765596145
GCGCTAG21452.6029738E-97.4301679
Produced by FastQC (version 0.11.5)