FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15503396
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA17150.041.876648145
CGCCGTT15250.036.15302145
GTAGATA86500.017.695677145
CGTGTAT38450.017.357706145
AGTGTTA92300.016.50511145
AGGGGGG193600.016.22495145
AAGGGGG209750.015.148617145
AAAAGGG257100.013.402702145
GTTCAGC101250.012.968339145
CCGTGTT50500.012.641312145
AAAGGGG280450.011.588398145
GCGTCGG38100.010.853023145
GCCGTAA16050.010.847684145
TCGTGTT69050.09.770574145
CGTATCA609750.09.410777145
CATTAAA566500.09.399331145
ATCATTA568950.09.295104145
CGTATAA9804.5474735E-118.882925145
AAAAAGG388800.08.844085145
GTATCAT627250.08.835955145
Produced by FastQC (version 0.11.5)