FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8937265
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA9100.019.916622145
AGGGGGG97250.016.996609145
AAGGGGG106250.013.919329145
AAAGGGG144850.012.212045145
TGTGCCG32350.011.877325145
AAAAGGG176650.011.614215145
CGTCGTT9953.4195182E-810.929122145
CGCCGTT6656.8650275E-510.90173145
GTAGATA64800.010.8521145
CGTATCA271250.08.846579145
GCCGTAA8407.1988686E-48.630536145
GCCGTAT305050.08.484265145
TCATTAA312050.07.9454594145
CCGTATC286050.07.7603664140-144
TCGCCGT338200.07.5883393145
CGCCGTA316300.07.471976145
AAAAAGG277100.07.299359145
TGGTCGC393450.07.259785145
GTATCAT296250.07.1945896145
CGAAAAG16204.7670965E-67.1688521
Produced by FastQC (version 0.11.5)