FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6977040
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG461700.09.342002145
AGGGGGG93800.09.119311145
AAGGGGG94900.07.9441967145
AAAGGGG113700.07.523231145
AAAAGGG121500.07.159585145
CGTCTTC233300.06.8047514145
TGCCGTC270200.06.599828145
TCGCGTA2450.00392862746.509321135-139
GCCGTCT243550.06.429075145
GTATGCC284250.06.3246145145
TATGCCG258100.06.1508603140-144
CTTGAAA193550.06.1423354145
CGTATGC291300.06.121777145
TCGTATG294150.06.111751145
CCGTCTT235250.06.0395722140-144
ATGCCGT263200.05.8609157140-144
CTGCTTG239050.05.852635145
TGCTTGA214400.05.612627145
CTCGTAT293650.05.5197773140-144
CGAGATT481050.05.5153584145
Produced by FastQC (version 0.11.5)