FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6977040
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA7051.0913936E-1116.458138145
CGTATCA128150.08.714689145
AGGGGGG78750.08.656196145
GCCGTAT152150.08.340957145
GGTCGCC194550.07.939592145
AAGGGGG75850.07.9354634145
CCGTATC139250.07.6451140-144
TCATTAA165400.07.5850844145
CGTGTAT9600.00268237137.5540276145
CGCCGTA152650.07.5535326145
AAAAGGG100850.07.5502825145
GTGGTCG212450.07.5095816145
TCGCCGT168150.07.4179068145
ATCATTA152800.07.185464140-144
CATTAAA163750.07.174366145
GTATCAT152450.07.1258507140-144
AAAGGGG91950.07.098075145
TGGTCGC194500.06.860378145
TATCATT164150.06.8476744140-144
TCGGTGG260250.06.715465145
Produced by FastQC (version 0.11.5)