FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9330320
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA16650.042.66821145
TCGTGTT46200.026.83161145
ACGTCCG49000.026.038092145
CGCCGTT15050.023.120506145
TGTACGG40600.019.283697145
GCGTCGG29000.014.24851145
TCGCCGG45150.014.129197145
GTAGATA85900.013.92462145
GGTGTAT50250.013.560771145
CGTGTAT42650.011.727963145
AGAGCGG74000.011.559602145
TCTCGGG81700.09.227921145
AGGGGGG132900.08.727454145
GGGAAAT125500.08.375617145
TGGTGTT118000.08.232189145
CGTATCA295050.08.157086145
GCCGTAA14458.7262015E-78.026842145
CGGTGGG97200.07.9055514145
TCGGAAT21453.1468517E-107.77308145
AAGAGTT81500.07.738455145
Produced by FastQC (version 0.11.5)