FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8501030
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG30000.021.51444145
ATTATCG10450.021.513283145
ATGCCGG30200.017.289677145
TCTCGTT38950.015.453681145
CATTATG62200.014.574073145
GGGGGGG763400.012.007599145
CACCGCG45800.09.817197145
AGGGGGG110950.09.020136145
CGTATGG8901.2907624E-48.963219145
ACACGTA20209.094947E-128.616307145
AAGGGGG109450.06.559651145
CATTATA89000.06.518704145
GTCTTCG33609.094947E-126.4750524145
TGCCGTG44150.06.406122145
TCGTATT22653.9995757E-76.403584145
GCCGTCT349200.06.292594145
CTTGAAA286900.06.167662145
GTATGCC431300.06.1372623145
CGTCTTA13000.007259576.1363573145
GAGCACC99100.06.0006943145
Produced by FastQC (version 0.11.5)