FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9939239
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG45350.032.46069145
ATGCCGG46500.025.263939145
CGTCTTA20500.018.748262145
GGGGGGG1240450.018.730623145
TGCCGTG58150.017.957745145
AGTCACC82450.017.76641145
TCACGAC16850.017.214684145
TCTCGTT45750.014.741133145
TCGGAAT27300.014.343994145
TCCATCG51650.014.0400505145
TTCCATG152150.013.3452145
AAGGGGG166600.013.18884145
AGGGGGG173200.012.435051145
ACACGTA26550.012.290994145
CGTATGG13650.011.687699145
AAAGGGG189850.010.92432145
TCGCGTT6707.3870484E-510.823413145
GTCTTCG46100.010.22472145
GAGCACC124900.09.579889145
GTCACGC29150.08.95577145
Produced by FastQC (version 0.11.5)