FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14038952
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAG21500.034.702484145
ACGTCCG63250.033.53793145
GATCGAA21650.032.434868145
GCGTCGG61250.028.54241145
CCGTATA21350.028.436691145
TGTACGG65200.027.710743145
CGTCGAG33050.027.22278145
ATCGGAG41350.027.06542145
TACGTCG23550.026.401392145
GTATCGT15050.025.273518145
TCGTGAA34350.022.146517145
GTCGTAT15200.022.13671145
GATCGGG50000.022.090513145
CGTCGGG46900.021.991005145
TACGTGC33900.021.793175145
ACGTCGA12300.020.814299145
AGAGCGG120750.019.930012145
ATCGGGA47600.019.669886145
CGTCGTA14750.019.340666145
GCCGTAA23500.019.29846145
Produced by FastQC (version 0.11.5)