FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13360249
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA43300.044.7646145
GATCGAA17050.042.470516145
GTATCGT15100.039.7204145
GCTATAT156950.035.18539145
GTCGTAT13850.033.79924145
ATCGGAG48150.033.419743145
CGTATGA27100.033.198048145
AGCGTAG26600.032.997143145
GCGTCGG71750.031.602142145
CGTCGAG40400.029.329885145
GCCGTAA34500.029.045454145
CGTCGGG58400.028.430843145
GATCGGG55850.028.157366145
CGTATCG27650.027.511543145
CGCCGTT22050.026.205667145
TCGTGAA33450.025.80255145
AGTGTCG36850.024.612795145
GTCGCGG44400.024.051746145
CGTATAG8750.023.405973145
ATCGGGA47450.022.968191145
Produced by FastQC (version 0.11.5)