FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9013112
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCG99150.11000639956543312TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14750.036.4597245
ATCGGAA16350.033.7516141
CGATGTA15500.033.24144733
TCGGAAG17050.032.5937422
CGTATGC16550.032.35817743
CCGATGT16100.032.28213532
CTCGTAT16400.032.24127641
ACCGATG16100.032.14238431
TCTCGTA15100.031.88765740
GTCACCG16900.031.15321728
CACCGAT16700.030.9875730
CGTCTGA17650.030.97602315
TATCTCG15600.030.8649338
ACACGTC17650.030.4661312
TCGTATG17400.030.38950742
ATCTCGT16550.028.9572839
TCACCGA18350.028.4464529
ACGTCTG19250.028.05075814
CGGAAGA20350.026.9765933
CACGTCT20050.026.8193113
Produced by FastQC (version 0.11.5)