FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25193968
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA55050.011.690421
CGTCCGA1853.0319588E-410.9438116
AAACGGG64150.010.8708987
ACGGGAT37150.010.8391149
AACGGGA52900.010.631268
GAAACGG65550.010.4670666
TATGCCG7650.09.70420245
CGGGATT35400.09.59562410
GGAAACG67050.09.5954445
CGAAACG5151.3460522E-109.1729695
AACGGGT14450.08.2510358
ACGCCCG16550.08.02017334
CGTATGC10900.07.636307243
ACCGCGC22350.07.24751331
CACCGCG23750.07.199196330
CTCGTAT10400.07.13818741
CGGGATA10400.07.13805910
GATCGGA7352.1736923E-97.0910351
ACAGGCG94800.07.071391618
GCGTGAG66300.06.853948622
Produced by FastQC (version 0.11.5)