FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9590365
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC273850.2855470047281829TruSeq Adapter, Index 2 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCG188430.19647844477243565TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA32800.041.9994661
TATGCCG25650.038.24757445
ATCGGAA56450.024.4263532
TCGGAAG57450.024.1969453
CGATGTA56850.024.14132534
CTCGTAT56300.024.14078942
CGTATGC56700.024.08853544
ACCGATG57700.023.90179432
TCTCGTA56950.023.8264941
CCGATGT57800.023.82226833
CACCGAT57900.023.81923731
TATCTCG57200.023.75995639
ACACGTC58250.023.74840713
TCGTATG57700.023.67179543
TCACCGA58300.023.57776830
GTCACCG58350.023.40236529
ATCTCGT58200.023.35268640
CGTCTGA59050.023.35048316
CGGAAGA60000.023.0561054
ACGTCTG60500.022.82802415
Produced by FastQC (version 0.11.5)