FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9245150
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11950.032.94882245
CCGTACG12950.029.18801332
CGTACGT12850.029.06482133
ACCGTAC14700.025.7132531
GTACGTA15000.025.64938434
CTCGTAT15000.025.64924443
TCTCGTA14950.025.58453242
TCGTATG15350.025.21098344
ATCGGAA16500.023.8735221
CACCGTA16050.023.69063630
GTCACCG16100.023.61706228
GATCGGA6200.023.598481
ACACGTC16650.023.51320312
ATCTCGT16350.023.25619541
TCGGAAG17200.022.8974632
CGTCTGA17450.022.43450515
CGTAATC17100.022.1047337
CGGAAGA18500.021.5308173
ACGTAAT18050.021.19062636
TACGTAA18450.020.97510535
Produced by FastQC (version 0.11.5)