FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23438615
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7850.012.60998945
CTCGTAT8900.011.88098641
CGTTGGA53250.011.3684791
AAACGGG57750.010.5571167
CGACGAT2201.26078E-410.2674951
CGTATGC10550.010.23607443
GAAACGG60300.010.1852886
AACGGGA48450.010.0761348
TCTCGTA9550.09.89444640
ACGGGAT37550.09.7657729
GGAAACG63550.09.4874015
CGGGATT36500.09.36870710
AACGGGT12400.08.8900138
ACAGGCG88700.08.16695718
GCGTGAG64500.08.09226622
ACCGCGC21550.08.03913731
TCGTATG13000.07.96084542
CGTGAGC67050.07.81806123
CACCGCG22750.07.615094730
CGTCTGA15550.07.52318715
Produced by FastQC (version 0.11.5)