FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13993857
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC7417455.300504357018941TruSeq Adapter, Index 10 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG6504154.64786084351155TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA808900.044.8368761
TATGCCG744500.043.24386645
ATCGGAA1529150.023.7101692
TCGGAAG1529450.023.6637653
CGGAAGA1534700.023.5432244
ACACGTC1534850.023.41718313
GCACACG1541000.023.36898611
CGTCTGA1536600.023.35767416
CACGTCT1539100.023.33790214
ACGTCTG1536800.023.3236415
CACACGT1541400.023.31475612
TCTCGTA1492100.023.3064941
TATCTCG1491700.023.29488239
ATCTCGT1494800.023.27181840
AGAGCAC1560950.023.145018
TCGTATG1540450.023.14456643
CGTATGC1538150.023.14114644
CTCGTAT1538850.023.14085242
GTATGCC1545450.023.11627245
GAGCACA1563950.023.1020559
Produced by FastQC (version 0.11.5)