FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4573205
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3700.032.23329545
CGGCTAC4550.026.70508232
ACGGCTA4600.026.4145231
TCTCGTA4750.025.58233640
CGTATGC4950.024.09331143
CGTCTGA5500.023.31438615
ACACGTC5400.022.91293512
CTCGTAT5150.022.72145741
AGTCACG5950.021.9337627
ATCGGAA5700.021.7381271
GTCACGG5800.021.33716828
TCGTATG5600.021.29746442
ATCTCGT5650.021.1089939
TCGGAAG6100.020.2864672
CGGAAGA6150.020.1215343
CATCTCG6200.019.23641838
TCACGGC6400.018.98543729
CACGGCT6850.018.06670430
ACGTCTG7000.017.99706814
CACGTCT6900.017.93186413
Produced by FastQC (version 0.11.5)