FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7789330
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG168690.2165654812416472TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA19600.041.8995131
CGTATGC9050.032.0718845
CCGTACG25200.032.05527533
CGTACGT25350.031.8660134
ACCGTAC26000.031.06856532
GTACGTA27150.029.75334735
TCTCGTA27200.029.6978943
CTCGTAT27050.029.69506544
CGTAATC27050.029.61397638
ATCGGAA27800.029.5404742
GTCACCG27550.029.31909629
TCGGAAG28250.029.308853
ACACGTC28100.029.14521413
TCGTATG28050.028.79684345
CGGAAGA29050.028.7340724
CACCGTA28200.028.72364231
ATCTCGT28100.028.42641642
ACGTAAT28400.028.28604537
TACGTAA28600.028.24505436
CGTCTGA29150.027.94101316
Produced by FastQC (version 0.11.5)