FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15233954
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGC369000.24222207839146687TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC46150.039.29545645
CTCGTAT49750.036.04469743
ATCGGAA51400.035.6552961
TCTCGTA50700.035.41333442
GTCACGA51200.035.1109828
TCGTATG51900.034.8117444
TATCTCG51500.034.73238440
TCGGAAG52950.034.2999462
ACACGTC53150.033.99112712
CGTCTGA53800.033.66409315
ACGAGTG53550.033.48602331
AGTCACG53800.033.41416527
ATCTCGT53750.033.36229741
CACGAGT54600.032.92447330
CGGAAGA56100.032.454253
ACGTCTG55750.032.44624714
CACGTCT59050.030.74729713
GCACACG60600.030.22073710
CGAGTGG60400.029.72560332
CACACGT64600.028.3146611
Produced by FastQC (version 0.11.5)