FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8142036
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG1056481.2975624278743056TruSeq Adapter, Index 7 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC219900.27007986700132497TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG136200.043.7956445
GATCGGA26850.042.877821
ATCGGAA163850.037.3043481
TCGGAAG164150.037.1171042
CGTCTGA163650.036.81805415
ACACGTC164250.036.79314412
CGGAAGA165800.036.7067833
CTCGTAT161600.036.65971441
CGTATGC163200.036.6051943
TCGTATG162850.036.44897542
ACGTCTG165500.036.4064914
CACGTCT166250.036.35051713
GCACACG166700.036.2924410
TCTCGTA143700.036.02838540
CACACGT168500.035.91853711
ATCTCGT144100.035.69328339
GTATGCC168850.035.4069744
CATCTCG146100.035.28167338
GCTCGTA6750.034.33352740
ACTCGTA9150.034.1805240
Produced by FastQC (version 0.11.5)