FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9933364
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19300.037.0740445
CTCGTAT22050.032.75642843
TCTCGTA21700.032.6614642
ATCGGAA23250.031.7460981
CGTCTGA23600.031.1755415
ACACGTC23750.031.16811612
TCGTATG23150.031.00557544
ATCTCGT23450.030.22404141
TCGGAAG24300.030.1848892
ACGTCTG25500.028.76442314
CACGTCT25750.028.7472913
CGGAAGA26000.028.4708733
AATCTCG27100.025.98682840
GCACACG28950.025.95829610
CACACGT29850.025.10025611
GATCGGA4950.023.6394631
AGTCAAC35900.019.67957133
GTCAACA39650.017.81832534
AGAGCAC42800.017.7159467
CAGTCAA43450.017.24387732
Produced by FastQC (version 0.11.5)