FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15299231
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG1992231.3021765603774464TruSeq Adapter, Index 10 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC349830.22865855153111947TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG237750.041.42722745
GATCGGA42000.039.8425981
ATCGGAA265500.037.7483831
TCGGAAG267800.037.3483052
CGGAAGA274900.036.3264053
ACACGTC276700.035.7965912
CTCGTAT275400.035.59260641
CGTCTGA277800.035.5900615
TATCTCG264350.035.5453738
TCTCGTA265450.035.49976740
CGTATGC277600.035.496743
TCGTATG277350.035.41513442
ACGTCTG280500.035.25550514
ATCTCGT267000.035.23470739
CACGTCT282000.035.1397713
GCACACG285000.034.9508510
CACACGT287200.034.59696211
GTATGCC288950.034.21120544
CACTAGC291350.033.77215630
GTCACTA292150.033.67967628
Produced by FastQC (version 0.11.5)