FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8897389
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCG112150.12604821481897668TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14050.035.7110945
CCGATGT16000.030.9362432
TCGTATG16200.030.83259242
CTCGTAT16150.030.78873441
TATCTCG15450.030.72707438
CGATGTA16050.030.69950933
ATCGGAA16900.030.6427821
CACCGAT16550.030.4519330
ACCGATG16700.030.44785931
CGTATGC16550.030.04459643
TCTCGTA16000.029.95240840
GTCACCG16850.029.77623228
ACACGTC17600.029.27453612
TCGGAAG17750.028.7817212
CGTCTGA17800.028.69248815
ATCTCGT17650.027.15232539
ACGTCTG19150.026.78711514
TCACCGA18750.026.75890729
CACGTCT20150.025.79313713
CGGAAGA20450.025.4204673
Produced by FastQC (version 0.11.5)