FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5242510
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGC54350.10367171450316737TruSeq Adapter, Index 14 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8100.034.44376443
TCTCGTA7400.033.74901242
CGTATGC8550.033.68354445
ATCGGAA8600.033.2489241
CCGTATC7750.032.80611837
TCCGTAT8000.031.78092836
ACACGTC8850.031.77812812
AGTTCCG9100.031.4010833
TATCTCG8150.031.19600140
CGTCTGA9100.031.1523415
TCGTATG9100.030.90598544
TCGGAAG9300.030.7293032
TTCCGTA9550.029.68584435
CGTATCT8950.028.40753438
ATCTCGT9000.027.99918241
CGGAAGA10400.027.262723
GTTCCGT10450.027.1291734
ACGTCTG10800.026.24873214
CACGTCT11350.024.97676713
GCACACG11450.024.7579210
Produced by FastQC (version 0.11.5)