FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27011932
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20500.032.70435345
CCGATGT24750.027.27272832
ACCGATG24900.026.9280631
CGATGTA25600.026.5429733
CGTATGC25900.026.23314343
TCTCGTA24150.026.08806840
CACCGAT26400.025.824230
CTCGTAT25850.025.76168841
ATCGGAA26900.025.1302971
TATCTCG25250.024.9505438
GTCACCG29150.023.8510828
TCGTATG28500.023.60312342
ACACGTC29600.022.80114212
TCGGAAG29800.022.7364862
TCACCGA30650.022.24335529
CGTCTGA31050.021.51998715
ATCTCGT29800.021.14097839
CGGAAGA35500.019.5885333
ACGTCTG35900.018.92519614
CACGTCT39700.017.39702213
Produced by FastQC (version 0.11.5)