FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14959124
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG8100.022.5083332
CGTATGC8350.022.09984845
CTCGTAT9950.018.54164143
GTTTCGG10250.018.00666233
TCTCGTA9700.017.39589942
ACACGTC11650.016.60775812
TCGTATG11150.016.3482844
ATCGGAA11850.015.5708531
GTCACGT12300.014.99848128
CGTCTGA13500.014.6651815
TCGGAAT10900.014.66138736
AGTCACG12550.014.34117627
TCGGAAG13250.014.2645172
ATCTCGT11600.014.15866441
TTCGGAA12650.014.05658435
TTTCGGA13550.013.45516234
GATCGGA5500.012.27371
CGTTGGA35000.011.8937991
ACGTCTG16250.011.76803814
TCACGTT16800.011.65060529
Produced by FastQC (version 0.11.5)