FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14229043
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7250.019.86489145
GATCGGA6150.019.4717791
CGTTTCG6400.017.57543432
CGTTGGA33650.012.4221
GTTTCGG9000.012.2483433
CTCGTAT10500.012.21602243
ATCGGAA10350.012.1727752
ACACGTC11250.011.79819313
ACGGGAT22900.011.6908019
TCTCGTA9900.011.59255643
AACGGGA31100.011.3571418
AAACGGG37700.011.2784717
TCGTATG11300.011.15202744
TCGGAAG11500.010.9550753
CGGGATT21250.010.9044810
GAAACGG38950.010.8587576
TTCGGAA10800.010.83171435
TCGGAAT9050.010.44042636
AGTCACG11900.010.20873627
GGAAACG41050.010.0292325
Produced by FastQC (version 0.11.5)