FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29499454
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17500.020.66897445
CTCGTAT17500.020.41249343
TCTCGTA17200.019.07046942
ATCGGAA19900.018.5040821
TCGTATG20500.017.42506444
TATCTCG18700.017.06084840
ACACGTC22400.016.7498112
TCGGAAG23800.015.675152
CGTCTGA24750.015.15906315
ATCTCGT22550.014.54655641
ACGTCTG29450.013.12122214
CGGAAGA30050.012.7858573
CACGTCT32350.012.50085313
CGTTGGA62850.011.3223941
GCACACG35750.011.06058310
ACGGGAT44200.010.0134889
AACGGGA58400.09.8484648
AAACGGG71450.09.7791167
CACACGT42300.09.71968411
GAAACGG73700.09.6937266
Produced by FastQC (version 0.11.5)