FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7705697
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7450.031.41220945
CTCGTAT7400.031.0160943
ATCGGAA8650.027.8607181
GTCACGT8700.027.15443228
TCTCGTA6900.027.06746742
AGTCACG9150.026.55665227
ACACGTC8900.026.54387712
TCGGAAG9450.025.7132512
TCGTATG9150.025.33013544
CGTCTGA9750.024.92175115
ATCTCGT7700.024.54621741
AACGATC7400.023.71603237
CGGAAGA10550.022.818843
ACGTCTG10750.022.18486414
CACGTCT11300.021.5035613
TCACGTG11800.019.82996729
CGTGAAA11700.019.80715232
CACGTGA12650.018.85324130
GCACACG13100.018.37712310
GAAACGA12650.018.31989735
Produced by FastQC (version 0.11.5)