FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10013443
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG132470.1322921596497828TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18450.037.31643345
GATCGGA9900.029.1014541
CGCCAAT25750.028.30976932
ATCGGAA27950.027.2191661
GTCACGC26200.027.0507928
CTCGTAT25800.026.95044141
TCTCGTA25700.026.7049440
CGTATGC26300.026.69395443
ACGCCAA27000.026.66580831
TATCTCG26050.026.51744838
ACACGTC27100.026.23348412
TCGGAAG29200.025.9725952
TCGTATG26800.025.776942
AGTCACG27600.025.67916327
CGTCTGA28300.025.6777215
ATCTCGT27000.025.5014739
ACGTCTG29950.024.03773314
CGGAAGA31500.023.4330843
CACGTCT31050.023.11358313
GCACACG34400.021.97406410
Produced by FastQC (version 0.11.5)