FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7312065
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGC91420.12502624087723507TruSeq Adapter, Index 22 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT11550.038.57014533
CCGTACG11600.038.40389332
CGTATGC12650.035.3952945
ACCGTAC13000.034.44210431
GTACGTA13300.033.8334634
CTCGTAT13500.032.4998143
CGTAATC12050.032.48943337
TCTCGTA12250.032.3263442
ACACGTC14200.031.84818612
ATCGGAA14250.031.6023161
TCGTATG14350.031.0451144
GTCACCG14550.030.77301428
ATCTCGT12800.030.58575841
TCGGAAG14850.030.3061662
CGTCTGA15100.029.94995115
CACCGTA15300.029.41179330
TACGTAA15400.029.07370835
CGGAAGA15500.029.0352633
ACGTAAT13500.028.99903736
ACGTCTG16000.028.12464114
Produced by FastQC (version 0.11.5)