FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8406769
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG125320.14907035033316607TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19850.038.42268845
GATCGGA9050.033.3633081
ATCGGAA27050.029.2383421
CTCGTAT26850.028.48972741
CGTATGC27050.028.2785843
TCGGAAG27850.028.2050612
TCGTATG27800.027.43522842
ACACGTC28450.027.28143912
TCTCGTA24150.027.20316340
CGGAAGA29150.027.0244143
CGTCTGA29550.026.266215
ACGTCTG30150.025.74318314
ATCTCGT25550.025.5366239
CACGTCT31200.024.94893313
GCACACG32250.024.55709510
GTATGCC32750.023.63173944
AATCTCG29500.022.4986438
CACACGT34800.022.43278911
ACTCGTA4550.018.7899640
AGTCACC43250.018.57113327
Produced by FastQC (version 0.11.5)