FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18321896
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG308010.16811033093954905TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41400.040.37756345
CTCGTAT44650.037.18660441
TCTCGTA42900.037.0817340
CGTATGC45700.036.4306743
ATCGGAA46700.036.1389961
ACACGTC47250.035.32937612
TCGGAAG47950.035.208572
TCGTATG47650.034.8925942
ATCTCGT46450.034.2947539
CGTCTGA49550.033.8719115
TCACGAT47200.033.74999634
ACGTCTG51850.032.36860314
CATCACG52200.032.1982732
CGGAAGA53550.031.603193
CACGTCT53450.031.44175313
ACGATCT53450.030.05612436
GCACACG56000.030.01003310
CACGATC54950.029.07187835
GTATGCC59200.028.42703244
CACACGT59200.028.38786711
Produced by FastQC (version 0.11.5)