FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12093412
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7600.027.2513081
CGTATGC8250.023.72681845
CGTCCCG10400.019.47182834
TCCCGAT11250.016.6013337
GTCCCGA11750.016.46770135
ATCGGAA13000.016.2702662
CCCGATC11900.015.88263938
CCGATCT12100.015.8067939
CTCGTAT12400.015.78722943
ACCCGTC13150.015.74335831
TCTCGTA12300.015.549705542
ACACGTC13100.015.45650713
TCGGAAG14500.015.0524323
CGTTGGA34050.015.0079671
TCGTATG13550.014.61214644
CACCCGT14200.014.41987230
TCACCCG14650.014.13053329
CCGTCCC15150.013.51649433
CCCGTCC15000.013.50158432
ATCTCGT14150.013.3576341
Produced by FastQC (version 0.11.5)