FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13341176
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGC177340.13292681244891755TruSeq Adapter, Index 13 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC25650.037.36673445
CTCGTAT27750.034.29575343
TCGTATG28350.033.56991644
ACACGTC28900.033.31592612
TCTCGTA25650.033.15503342
ATCGGAA29450.032.8713761
ATCTCGT26650.032.24886741
TCGGAAG30100.032.2172432
CGTCTGA30650.031.2669215
CGGAAGA32550.029.9305363
ACGTCTG32350.029.62383714
CACGTCT34050.028.27695513
GCACACG35400.027.07190510
AATCTCG32950.025.87769540
CACACGT38250.025.40728611
AGTCAAC47900.020.57264533
GATCGGA5300.019.6309531
GTCAACA53750.018.2498634
AGAGCAC55250.017.9156637
GAGCACA59600.016.8345348
Produced by FastQC (version 0.11.5)