FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362385
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC95390.2836974350052121TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10350.041.536291
CTCGTAT10200.040.145642
TCGTATG10300.039.97427443
CGTATGC10250.039.9497744
ATCGGAA10850.039.8260652
TCTCGTA10800.037.9152941
CGTCTGA11300.037.43449416
CGGAAGA11350.037.0707934
TCGGAAG11700.036.5474623
ACACGTC11650.036.5029913
ATCTCGT11900.035.35585840
ACGTCTG12000.035.06331315
CATCTCG12050.034.91574539
GTATGCC12750.032.4694145
CACGTCT13200.032.3871114
GCACACG13250.032.09317411
CACACGT13600.031.59999512
CTTAGGC15400.027.32043633
ACTTAGG16400.025.92893632
CACTTAG17200.024.46131931
Produced by FastQC (version 0.11.5)