FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11723378
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4800.024.37216645
TCTCGTA6050.018.22102240
CTCGTAT6850.017.73516341
GTCACGA7050.017.551328
CGATCAG6650.017.25657532
ATCCGCG800.001713494716.8729676
CGTATGC7700.016.0695643
TCGTATG8050.015.92982442
ATCGGAA7950.014.77557851
ACACGTC8550.013.94568712
ACGATCA8900.013.39960431
TCGGAAG8950.013.3224162
TCACGAT9050.013.17520829
AGTCACG9700.012.98829127
ATCTCGT9050.012.18085239
CGTCTGA9800.012.166915
CGGAAGA11750.011.4879773
CGTTGGA23700.011.151821
ACGTCTG11250.010.598721514
GAAACGG29400.010.1008236
Produced by FastQC (version 0.11.5)