FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12887070
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGC424880.3296948026199904TruSeq Adapter, Index 14 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC50600.038.95210345
TCTCGTA58000.033.55550442
CTCGTAT58600.033.2120643
TATCTCG58850.033.10907740
CCGTATC59050.032.99642637
ATCGGAA61350.032.9136431
TCGTATG59600.032.88131744
AGTTCCG60100.032.68200333
TCCGTAT60650.032.57111436
TCGGAAG62350.032.264672
CGTATCT60450.032.23236538
ATCTCGT60950.031.96831941
TTCCGTA62300.031.63624635
ACACGTC63200.031.5417312
GTTCCGT62450.031.48820534
CGTCTGA63350.031.21843715
CGGAAGA65050.031.0634843
ACGTCTG65850.030.06739214
CACGTCT69050.028.9672313
GCACACG69450.028.70409210
Produced by FastQC (version 0.11.5)