FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19162404
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG2437551.2720481208933911TruSeq Adapter, Index 6 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC421580.2200037114341186TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG279450.043.16676745
GATCGGA51600.040.6394541
ATCGGAA326150.037.710281
TCGGAAG328850.037.343522
CGCCAAT325950.037.1033932
ACACGTC328600.037.0082912
GTCACGC326600.036.920328
CGTATGC325450.036.89964743
CTCGTAT324200.036.88231741
ACGCCAA328050.036.845331
CGGAAGA332600.036.8412743
CGTCTGA329200.036.83862715
TCTCGTA311000.036.791140
TCGTATG326800.036.76146742
TATCTCG310250.036.74951638
AGTCACG329050.036.6248927
ACGTCTG332850.036.40762314
ATCTCGT313250.036.3904839
CACGTCT335050.036.2555613
GCACACG338550.036.03413810
Produced by FastQC (version 0.11.5)