FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11058585
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1423951.287642135047115TruSeq Adapter, Index 8 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG1185991.0724608980262844TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA157850.044.0539671
TATGCCG156100.043.183245
ATCGGAC4300.031.9354521
AATCGCG2150.031.39615238
CGCGTAT1400.030.53732541
ATCGCGT1950.030.00158539
TCGCGTA1350.030.00158140
CGGACGA2200.029.6621323
TCGGACG3800.029.6084332
TACGCCG1058.54925E-1125.71389845
ATCGGCA6250.022.691971
ATCGGAA320500.022.6523571
TCGGAAG320000.022.4320892
CTCGTAT306300.022.33232742
TCGTATG308850.022.24265343
TCTCGTA307750.022.23431641
CGTATGC309700.022.1651744
CGTCTGA312250.022.09114316
ATCTCGT310200.022.03694740
CGGAAGA316550.022.001153
Produced by FastQC (version 0.11.5)