FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66924071
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGC2287400.34179032533750076TruSeq Adapter, Index 15 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC270350.039.74201645
CTCGTAT317350.033.54504443
ATCGGAA325600.033.3624531
TCTCGTA320150.033.27307542
TCGTATG326300.032.8644944
TCGGAAG332750.032.6220172
ACACGTC333000.032.44816612
ATCTCGT331900.032.0897441
CGTCTGA338000.032.0147515
GATCGGA73950.031.6925831
CGGAAGA345150.031.4630583
ACGTCTG351100.030.8202414
CACGTCT359150.030.22339413
AATCTCG358900.029.69455740
GCACACG370300.029.34348110
CACACGT377850.028.6857511
ACATGTC451650.023.90546231
AGAGCAC466650.023.4536157
TCTGAAC482650.022.64840717
AGCACAC487850.022.5035869
Produced by FastQC (version 0.11.5)