FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10332168
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21050.035.5712945
GATCGGA9400.034.477491
ATCGGAA28550.027.1627771
CTCGTAT28000.026.26014943
TCTCGTA28100.026.24671742
AACGATC28050.025.81277537
TCGGAAG29250.025.6757932
TCGTATG29450.025.4253244
ACACGTC30100.025.2495412
CGTCTGA29850.025.08436815
GTCACGT30450.024.44264228
ATCTCGT30450.024.29495241
AGTCACG28900.024.04174627
CGGAAGA31650.023.7288133
ACGTCTG32000.023.39901214
AAACGAT31600.023.19755436
CGTCACG2150.023.00856827
TCACGTG32750.022.8633829
TGAAACG32300.022.48608234
CACGTCT34000.022.48553713
Produced by FastQC (version 0.11.5)