FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3701054
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG70100.1894055044860194TruSeq Adapter, Index 19 (97% over 40bp)
CCCAAAACCTTATCTAATAATAACCAAAACCATCCACCACCACCTTCCAAT41580.11234637484349053No Hit
GAGTTTTTATTAGGGAGGTTTTTTGAATTGGAGGTAGAATTTTGTTTTTTA41460.11202214288146026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG1350.041.6628156
CGACGCT1100.040.9152642
ACGGCGA1400.040.174867
GATCGGA9700.038.9960251
ATCGGAA10300.038.0077362
GTCACGT9700.037.57384529
ACACGTC11400.037.49653213
TCGGAAG10550.036.8938183
AGTCACG10050.036.71500828
AACGATC9650.036.60283338
CGTCTGA11750.036.37961616
ACGTCTG12350.034.61218315
TCTCGTA10300.034.0841943
TCACGTG10700.033.43148830
ACGACGC1350.033.33520541
CTCGTAT10600.033.33184444
CGGCGAC1700.033.0851758
TCGTATG10750.033.0760945
ATCTCGT10700.032.8086842
CACGTGA11100.032.2267531
Produced by FastQC (version 0.11.5)