FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15914526
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGC2417471.5190336174636931TruSeq Adapter, Index 18 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG476850.2996319211769172TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC295750.041.0856345
GATCGGA56700.040.6887471
ATCGGAA330650.037.367751
TCGGAAG334200.036.922072
CGGAAGA341600.036.1090663
ACGTCCG343800.035.36638631
CGTCCGC344650.035.3122432
GTCCGCA345300.035.1545633
ACACGTC347900.035.1440212
TCCGCAC344850.035.12867734
CGTCTGA347900.035.11815615
CTCGTAT344700.035.11298843
TCGTATG346700.034.98290644
ACGTCTG350250.034.87610614
GTCACGT348450.034.8621528
CCGCACA347050.034.7962335
AGTCACG348950.034.79285427
TCTCGTA324800.034.72229842
TCACGTC351050.034.67443529
CACGTCT353200.034.6039213
Produced by FastQC (version 0.11.5)