FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6793694
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG222360.3273035258873891TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28050.042.35101345
ATCGGAA29500.040.8137441
TCGTATG29350.040.3277842
CTCGTAT29550.039.9022441
ACACGTC30100.039.839512
CGTATGC30000.039.6040343
TCGGAAG30450.039.5340042
CGTCTGA30500.039.0968715
TCTCGTA26900.038.98137340
ATCTCGT26950.038.7420639
ACGTCTG31850.037.65162714
CGGAAGA32350.037.2810753
CACGTCT33500.036.06579213
GCACACG34450.034.74442710
GTATGCC34600.034.333744
CACACGT35300.033.90780611
AATCTCG31100.033.499738
GTCACAC41000.029.24996228
AGAGCAC44600.027.191877
AGCACAC45500.026.7020849
Produced by FastQC (version 0.11.5)