FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12716659
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC462390.3636096556493337TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA54550.042.167261
CGATGTA62150.036.2737234
CTCGTAT61800.036.22815742
CCGATGT62750.036.07058333
CGTATGC62400.036.0224944
ACCGATG62600.035.9415132
CACCGAT62850.035.9059431
ATCGGAA64450.035.7883452
TCGTATG63000.035.71597743
GTCACCG63250.035.64371529
TCTCGTA62750.035.57210541
TATCTCG62650.035.55565339
ACACGTC63650.035.48859813
TCGGAAG65200.035.342153
ATCTCGT63600.035.09641340
TCACCGA64550.034.96072430
CGGAAGA67150.034.31574
CGTCTGA65700.034.28109716
ACGTCTG68200.032.95614215
CACGTCT69450.032.4925614
Produced by FastQC (version 0.11.5)