FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7866978
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6900.032.61041345
CGTCCGC7200.030.31236332
GTCCGCA7150.030.20984633
CTCGTAT7250.030.10503443
ACGTCCG7050.029.99986531
TCTCGTA6850.027.92117942
TCGTATG8450.026.36233544
TCGGAAG8650.025.7500212
TCCGCAC8450.025.56250234
ATCGGAA8800.025.3392811
ACACGTC9200.023.96606312
CGCACAT8300.023.8557736
CGTCTGA9500.023.4460715
GTCACGT9650.022.38331828
AGTCACG10200.022.05858427
CCGCACA10250.021.9515435
ATCTCGT9050.021.63056241
CGGAAGA10450.021.5299073
CATCTCG9050.021.3820740
TCACGTC10700.020.60738429
Produced by FastQC (version 0.11.5)