FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227832
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG587881.8212843791126678TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA62600.044.3988531
ATCGGAA63250.044.0465932
TCGGAAG63550.043.764453
TCTCGTA62750.043.38736343
ACACGTC63750.043.23486313
CTCGTAT63000.043.17947844
CGTCTGA63800.043.16571416
TCGTATG62850.043.1393345
TATCTCG63500.042.98121641
CGGAAGA64650.042.9468734
ATCTCGT63450.042.8377842
ACGTCTG64500.042.62748315
CACGTCT65150.042.3403314
GCACACG65250.042.27609311
CACACGT65250.042.17264612
GTCACAC68400.040.0647729
AGAGCAC69900.039.4953168
TCTGAAC69900.039.46251318
CTGATAT70400.038.92656335
ACTGATA70250.038.91359734
Produced by FastQC (version 0.11.5)