FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005310445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14659844
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG224555715.317741443906224TruSeq Adapter, Index 8 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGCCG874520.5965411364541123TruSeq Adapter, Index 8 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC530650.36197520246463744TruSeq Adapter, Index 8 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAGCTCGTATGCCG300850.20522046482895723TruSeq Adapter, Index 8 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAACCTCGTATGCCG229770.15673427357071468TruSeq Adapter, Index 8 (98% over 51bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG162950.11115397953757215TruSeq Adapter, Index 8 (98% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)