FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12122361
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG805090.664136301501003TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG97250.042.92193645
GTCACGA109100.038.12516428
ATCGGAA111250.038.1146241
CTCGTAT108450.038.10821541
CGTATGC109850.037.94991343
CGATCAG109550.037.9279632
TCTCGTA100750.037.89134240
TCGTATG110250.037.75100742
TCGGAAG112450.037.6110882
CGTCTGA112100.037.54697415
ACACGTC111650.037.53711712
TCACGAT111200.037.42540729
ACGATCA111500.037.26418731
AGTCACG111600.037.25095427
ATCTCGT102450.037.1747639
CGGAAGA114200.037.0347373
ACGTCTG114050.036.78665514
CACGTCT116900.036.02451313
CACGATC116700.035.79650530
GCACACG117600.035.618210